Uptake of [3H]-nicotine and [3H]-noradrenaline by cultured chromaffin cells
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Deposition of [3H]cocaine, [3H]nicotine, and [3H]flunitrazepam in mouse hair melanosomes after systemic administration.
Microautoradiography was employed to show that association of drugs from the serum directly with forming hair pigment is a primary pathway of deposition into the hair. After systemic administration of [3H]flunitrazepam, [3H]nicotine, and [3H]cocaine, association of all three drugs with melanin in the forming hair was observed within minutes of dosage. Sebum was determined to be an insignificant...
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The satellite cell (6) is a mononucleated cell that is wedged between the multinucleated muscle syncytium and the basal membrane of the myofiber. These cells are described in skeletal muscle of amphibians (6, 7) and mammals (3, 12-14). From data in the literature it is clear that satellite cells are most abundant during embryonic life, though it is not obvious that they participate in the norma...
متن کاملA desensitized form of neuronal acetylcholine receptor detected by 3H-nicotine binding on bovine adrenal chromaffin cells.
A nicotinic acetylcholine receptor (AChR) on bovine adrenal chromaffin cells in culture has previously been identified using the alpha-neurotoxin n-Bgt and the monoclonal antibody mAb 35. Here, we report that the cells have 2 classes of high-affinity binding sites for 3H-nicotine, one being associated with the AChR and the other being associated with the alpha-bungarotoxin binding component tha...
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Three radioligands have been commonly used to label putative nicotinic cholinoceptors in the mammalian central nervous system: the agonists [3H]nicotine and [3H]acetylcholine (C3H]ACh-in the presence of atropine to block muscarinic receptors), and the snake venom extract, [1251]-a-bungarotoxin([‘251]BTX), which acts as a nicotinic antagonist at the neuromuscular junction. Binding studies employ...
متن کاملElectrically Evoked Release of [3H]Noradrenaline from Mouse Cultured Sympathetic Neurons
Cultured neurons from the thoracolumbar sympathetic chain of newborn mice are known to possess release-inhibiting a2-autoreceptors. The present study was carried out in a search for release-modulating heteroreceptors on these neurons. Primary cultures were preincubated with [H]noradrenaline and then superfused and stimulated by single pulses, trains of 8 pulses at 100 Hz, or trains of 36 pulses...
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ژورنال
عنوان ژورنال: British Journal of Pharmacology
سال: 1984
ISSN: 0007-1188
DOI: 10.1111/j.1476-5381.1984.tb10751.x